Iron (Fe) is essential cofactor in various physiological processes such as the operation of the photosynthetic functions. In Planta, Fe content of leaves also serves as Fe source during senescence processes. Retranslocation of Fe is connected to the alteration in the regulation of chloroplast Fe homeostasis. Our data (Pham et al, 2020, Sági-Kazár et al, under peer-review) indicate that regulation of the chloroplast Fe homeostasis and senescence processes are in a close interaction. The understanding they way and reasons of Fe remodelling may provide a basis for the development of better foliar Fe application and biofortification techniques. With the help of single point μXANES we could determine any possible chemical modifications in the Fe content of chloroplasts as well as that of mesophyll cells in the areas of interest. Data are essential for the proper interpretation of ou molecular biological information.