Structural and behavioral characterization of transmembrane proton pumps in novel biomembrane mimics

DOI

Our team has recently developed novel biomimic membranes (BMM)consisting of a polymer-supported lipid bilayer with incorporated transmembrane proton pumps and ion channels. We have incorporated the proton pumps NhaA and bacteriorhodopsin, and the ion channel VDAC. We have shown they are functional by measuring ion transport across the samples. These BMM samples are completely novel and have great potential in bionanotechnology applications. We recently measured the VDAC samples on FIGARO leading to insightful results. We require the use of neutron reflectivity to obtain the detailed structure and behavior of our proton pump (NhaA and bacteriorhodopsin) samples. Particularly, we want to understand: the mechanism of proton pump incorporation; change in bilayer thickness after protein incorporation; effect of pH on the BMM-NhaA structure; effect of light on the BMM-bacteriorhodopsin structure; and location of deuterated water in the protein channels. The outcomes from usage of this beam-time will be to develop a fundamental understanding of biologically-inspired membrane transport and to utilise this knowledge to create novel energy sources.

Identifier
DOI https://doi.org/10.5291/ILL-DATA.8-02-659
Metadata Access https://data.ill.fr/openaire/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=10.5291/ILL-DATA.8-02-659
Provenance
Creator Stidder, Barry; Martin, Donald; Watkins, Erik; Fragneto, Giovanna; Alcaraz, Jean Pierre
Publisher Institut Laue-Langevin
Publication Year 2013
Rights OpenAccess; info:eu-repo/semantics/openAccess
OpenAccess true
Representation
Resource Type Dataset
Size 1 GB
Version 1
Discipline Particles, Nuclei and Fields