Stream benthic macroinvertebrates and a set of environmental variables have been collected for a period of 7 consecutive years (2016-2022, from April/May to September/October, during the glacial melting season) along a longitudinal transect of the Saldur stream, a near pristine glacier-fed stream located in South Tyrol, Italy (46°N, 10° E), part of the International Long Term Ecological Research (ILTER) network (site code: LTER_EU_IT_100). From year 2016 on, a small run-of-river (ROR) hydropower plant (max. 3,200 kW/year) started to operate on the stream, located in close proximity to site Saldur_2A. The potential impact on the riverine ecosystem was largely investigated, and resulted to be negligible. Extensive details on this topic can be found reading the scientific publications: Scotti et al. (2022; doi:10.3389/fenvs.2022.902603) and Scotti et al. (2022; doi:10.3389/fenvs.2022.904547).Organisms were collected through Surber samplings (0.0506 squared metres, mesh size 500 µm) in 3 sites at different elevation and distance from the glacial source: Saldur 1 (2,030 m a.s.l.; 4.962 km), Saldur 2A (2,016 m a.s.l.; 5.325 km), Saldur 3 (1,645 m a.s.l.; 11.123 km). In all sites, three different sub-samples were collected, and the main substrates present at each site were sampled (macrolithal - rocks of 20-40 cm, and mesolithal - rocks of 6-20 cm). Faunal nomenclature fully complies with Fauna Europaea standard (https://fauna-eu.org). Contextually, a set of environmental variables was measured (as spot-measurement) during the macroinvertebrate samplings: suspended solids (mL/L); specific conductance at 25° C (mS/cm), water temperature (°C), calculation of the bottom component of the Pfankuch index. Whilst only in 2018 turbidity (NFU) was measured instead of suspended solids, and all the measurements were conducted using a multiparameter meter (HI9829, Hanna Instruments), for all the remaining years suspended solids were measured after 30 minutes of water sedimentation in a Imhoff cone, and specific conductance through a portable conductimeter (Cond 7, XS Instruments). More methodological details can be found in Scotti & Bottarin (2021; doi:10.1038/s41597-021-00887-x) and Scotti et al. (2019; doi:10.3897/BDJ.7.e33576). A similar dataset , with coarser taxonomic resolution, is available on PANGAEA covering the sampling on the same sites during years 2010-2015: Bottarin & Scotti (2019; doi:10.1594/PANGAEA.897915). Together with the present dataset, the time series now covers 13 years.