The depletion of dissolved oxygen in lakes (hypoxia) is an ongoing phenomenon that put under risk ecological systems and impact sedimentary environments. This phenomenon is driven by the increasing anthropogenic pressure on such environments. This dataset contains high-resolution sedimentological, geochemical and biological depth series of selected short sediment cores from lake Tiefer See (NE Germany). Those cores cover the recent transition from homogeneous to laminated sediments (~100 years ago), a transition that reflect the onset of hypoxic conditions in the lake. The cores were taken from different locations and water depths across the lake and allow to trace the spatiotemporal evolution of hypoxia spread in the lake.