RNA-seq was used to compare differential gene expressions for Aspergillus flavus wild type strain and ASPES transcription factor deletion strains.The goals of this study are to explore the aflatoxin regulation pathway in A. flavus. Overall design: Examination of differential gene expressions by RNA-seq in Aspergillus flavus wild type strain under both YES and YEP, and two APSES transcription factor mutant strains(AfRafA knoutout strain and AfStuA)