Total bacteria and archaea number with different nematodes and substrates conditions

DOI

Data contain the quantitative polymerase chain reaction (qPCR) numbers of total bacterial and archaeal 16S ribosomal RNA (16S rRNA) genes from 3 different nematode treatments and two different substrates conditions in the natural soil. In first treatment condition there was no addition of nematode, in second treatment bacterivorous nematode (Acrobeloides buetschlii) was added and in third treatment there was the addition of fungivorous nematode (Aphelenchoides saprophilus). Meanwhile, two different substrate conditions were: with and without maize litter. The natural soil for these experiments were collected from Dikopshof agricultural site (50°48′21″ N, 6°59′9″ E), Bonn, Germany. These qPCR numbers were collected in June and July, 2023 to look at the effect of nematode grazing in the bacterial and archaeal populations. The qPCR was performed using primer set of 1389F (5′-TGYACACACCGCCCGT-3′) and 1492R (5′-GGYTACCTTGTTACGACTT-3′) with 20 µl reactions on a qTOWER3G (Analytik-Jena, Jena, Germany).

Identifier
DOI https://doi.org/10.1594/PANGAEA.974253
Related Identifier IsSupplementTo https://doi.org/10.1111/1462-2920.70007
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.974253
Provenance
Creator Tamang, Mandip; Huber, Katharina; Neumann-Schaal, Meina; Pester, Michael ORCID logo
Publisher PANGAEA
Publication Year 2025
Funding Reference German Research Foundation https://doi.org/10.13039/501100001659 Crossref Funder ID 441899689 https://soilsystems.net/ SPP 2322: Systems ecology of soils – Energy Discharge Modulated by Microbiome and Boundary Conditions (SoilSystems); German Research Foundation https://doi.org/10.13039/501100001659 Crossref Funder ID HU3067/1-1 ; German Research Foundation https://doi.org/10.13039/501100001659 Crossref Funder ID NE 2192/4-1 ; German Research Foundation https://doi.org/10.13039/501100001659 Crossref Funder ID PE2147/6-1 ; German Research Foundation https://doi.org/10.13039/501100001659 Crossref Funder ID RU 780/22-1 ; German Research Foundation https://doi.org/10.13039/501100001659 Crossref Funder ID UR198/7-1
Rights Creative Commons Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Representation
Resource Type Dataset
Format text/tab-separated-values
Size 405 data points
Discipline Earth System Research
Spatial Coverage (6.986 LON, 50.806 LAT)
Temporal Coverage Begin 2021-08-01T00:00:00Z
Temporal Coverage End 2021-08-31T00:00:00Z