Effect of oxidation on lipid raft formation: an off-specular neutron reflectivity study

DOI

The very heterogeneous, but non-random, arrangement of lipids and proteins in cell membranes, known as rafts are of major scientific interest. Lipids in cell membranes are continually under attack from reactive oxygen species with the result that new, oxidized species of lipid are continually being formed. Although oxidized lipids have implications in cell apoptosis, cancer and aging, very little work has been performed to understand the effect of oxidative lipid damage on raft formation. We propose, therefore, to use specular and off-specular neutron reflection in combination with contrast variation to probe the effect of the presence of oxidized lipid on the persistence and size of rafts formed. These studies will allow a better understanding of the factors leading to the formation of microdomains in biological membranes and in particular the influence of oxidation thereupon.

Identifier
DOI https://doi.org/10.5286/ISIS.E.61780290
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/61780290
Provenance
Creator Dr Katherine Thompson; Dr Ivana Knyght; Professor Jayne Lawrence; Dr Robert Dalgliesh; Dr Nina-Juliane Steinke; Professor Martin King
Publisher ISIS Neutron and Muon Source
Publication Year 2018
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Chemistry; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering; Natural Sciences
Temporal Coverage Begin 2015-06-30T07:00:00Z
Temporal Coverage End 2015-07-04T07:00:00Z