Our manuscript aimed to use RNA sequencing technology to study the transcriptomics responses and mechanisms of this species to temperature fluctuations. We focused on both a short term and a more sustained exposure to heat stress. We report significant effects of temperature on the gene expression of Temora. Overall design: Eight replicates were kept for four days at 14 °C and the other eight were acclimatized to 17 °C over the course of 24 h and kept at this temperature for the remaining three days. Subsequently at day four, four randomly selected replicates were moved for 24 hours to 17 °C from the 14 °C group and vice versa. This resulted in four treatment groups (1) a group exposed to 14°C for five days, (2) a group exposed to 17°C for five days (below referred to as the 'sustained exposure' group), (3) a group exposed to 14°C for four days and then to an additional acute exposure of 17°C (below referred to as 'acute exposure' group), and (4) a group exposed to 17°C for three days and then to an additional recovery exposure of 14°C (below referred to as 'recovery exposure' group). Only 3 replicates for each treatment group were sequenced.