Identification and characterization of diketopiperazines synthetic gene in Shewanella baltica and its role in quorum sensing

Much attention has been focused on the production pathway of quorum sensing (QS) signal molecule and action mode of quorum sensing inhibitor (QSI), which is also the hotspot in the field of food preservation. In this study, Shewanella baltica was the specific spoilage organism (SSO) in large yellow croaker at 4 °C storage, and SB-19 strain exhibited the highest spoilage capacity and produced the highest QS signal molecule of autoinducer-2 (AI-2) and diketopiperazines (DKPs), which was consisted of Cyclo-(L-Pro-L-Leu) and Cyclo-(L-Pro-L-Phe). After genomic sequencing and local blast, sb1370 gene was screened to be related to produce DKP in the SB-19 strain. Then knock-out strain and rescue strain of sb1370 gene were prepared, and their AI-2 and DKPs contents implied that sb1370 gene was involved in the synthesis process of DKP thorough cyclodipeptide synthase (CDPS) pathway, but did not in AI-2. Furthermore, fish fillets and mutation strains were treated by resveratrol, which strongly inhibited lipid oxidation of fillet, QS phenomenon, and sb1370 gene expression while not interfere with bacterial growth, suggesting resveratrol was an ideal QSI to inhibit DKP sb1370 gene and then QS phenomenon in S. baltica, resulting in delaying the process of fish spoilage during chilling storage.

Identifier
Source https://data.blue-cloud.org/search-details?step=~012C2ABAB6874D57F37A83CABE366C8D02AFB07CBD6
Metadata Access https://data.blue-cloud.org/api/collections/C2ABAB6874D57F37A83CABE366C8D02AFB07CBD6
Provenance
Instrument Illumina HiSeq 2000; ILLUMINA
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Temporal Point 2013-08-31T00:00:00Z