In order to improve understanding of past vegetation, functional diversity and climate change reconstruction in the past, we provide here the first dataset of combined pollen surface samples and vegetation plots from Tajikistan and Uzbekistan, known as the Tajikistan and Uzbekistan Surface Data Base (TUSDB). The database contains surface data (pollen surface sample, associated vegetation plots), environmental database extractions for each site (mainly climate, vegetation description and plant functional traits), results from modelled variables relevant for paleoclimate and palaeoenvironmental studies (Davis Indices, pollen R-values, pollen-based plant vegetation traits and biome-scores) and an application of these methods to an available Holocene pollen sequence from the Aral Sea (climate, biome and functional diversity reconstructions). This database may enhance our understanding of past climate and vegetation changes in Arid Central Asia (ACA).