The goal of this proposal is to assess the bone quality of osteosarcoma (OS) resected human tissue, by using xray scanning micro-diffraction at a spatial resolution down to 1 micron. This will allow to visualise and evaluate the bone microarchitecture, bone mineral platelets and collagen fibrils of osteosarcoma tissue. The bone microarchitectures and bone quality of OS will be compared with the healthy control bone and with histological data of the same subject. Due to its rarity, osteosarcoma is still lacking a reliable and precise characterization of the bone quality morphology as well as microarchitecture information at the micron scale. The results of this proposal will help to tackle this gap and will get a better understanding of bone quality, microarchitecture changes in OS tissue.