This BAG application builds on the development of single particle cryoEM in the Italian Structural Biology community, as in Italy there is no top-end instrument isopen to rhe structural biology community. The groups in this application have acquired know-how in cryoEM through international collaborations or by acquiring medium-level in-house equipment. Access tothe CM01 beamline at the ESRF since August 2020 has allowed the development of projects from groups that do not have in-house equipment that resulted inpaper in publication on Nucleic Acid Research NAR. We now have six screening microscopes in Italy and two more are being funded, making the access to CM01 more demanded and relevant for high-resolution data and, consequently high profile publications