The "UT-GPCR003 Fluorescence anisotropy and microscopy measurements experimental metadata of ligand binding to M4 muscarinic receptors" dataset contains the raw fluorescence anisotropy data for ligand binding to M4 muscarinic receptors on budded baculovirus surface as well as processed data for fluorescence anisotropy measurements and microscopy measurements connected with the experimental design metadata. The dataset contains competition binding experiments between reporter ligands (UR-CG072 and UR-MK342) and unlabeled ligands, kinetic experiments of fluorescence ligand binding and fluorescence ligand saturation experiments.