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The Influence of Different High Fidelity DNA Polymerase on 16S rRNA Sequencing
Assessing differences due to high fidelity DNA polymerase to downstream 16S rRNA gene survey results. Variations in number of OTUs and chimera prevalence were found to be high... -
Chinook salmon intestinal microbiome 16S survey
This project aimed to survey the intestinal bacteria of adult Chinook salmon (Oncorhynchus tshawytscha) aquacultured in New Zealand. The microbiota of 30 salmon from a single... -
Comparative analysis of amplicon and metagenomic sequencing methods reveals k...
Background: The interplay between hosts and their associated microbiome is now recognized as a fundamental basis of the ecology, evolution and development of both players. These... -
Evaluation of genus-specific primers for the study of Fusarium communities in...
Fusarium is a large and diverse genus of fungi with various ecological characteristics, from saprophytes to mycotoxin producers, phytopathogens but also biocontrol agents and... -
The effect of periodic disturbances as dilutions and carrying capacity on the...
In this study a marine microbial community was collected from 70 m depth in the Trondheim fjord in Norway and used to inoculate twelve bioreactors (volume 250 mL). The... -
Evaluation of PCR conditions for characterizing bacterial community with full...
Our study concerns the reliability of techniques for obtaining accurate and quantitative full-length 16S rRNA amplicon sequencing data on bacterial community structure with...