A study of protein adsorption via chelating lipids within a solid supported lipid layer

DOI

The crystallisation of membrane proteins is of great interest, not least, to the pharmaceutical industry. We have recently begun a project aiming to investigate the role played by surfaces in the nucleation and growth of protein crystals. The first step of this process is to study the growth of two-dimentional protein crystals on a solid supported lipid bilayer, prepared according to well established procedures. In this proposal we aim to characterise the adsorption of a protein as it binds to a chelating lipid within a solid supported lipid layer. This will help us to understand how the process of 2D crystalisation via such chelating lipids, which is well known at the air-liquid interface, can be successfully transferred to the solid-liquid interface.

Identifier
DOI https://doi.org/10.5286/ISIS.E.24089628
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24089628
Provenance
Creator Professor Karen Edler; Dr Thomas Arnold; Dr Hanna Wacklin-Knecht; Dr Gavin Hazell
Publisher ISIS Neutron and Muon Source
Publication Year 2015
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2012-10-10T15:38:13Z
Temporal Coverage End 2012-10-15T08:05:44Z