The Mechanism of Bam Mediated Outer Membrane Protein Folding

DOI

The proteins of the outer membrane of Gram-negative bacteria are critical for bacterial survival,from providing basic physiological functions, to impairing virulence and multidrug resistance. The Bam complex has been found to be essential for folding these proteins into the outer membrane.The process by which this is achieved is unknown. The structure of the complex and its individual components are now known. We aim to continue our investigation of the Bam complex by studying BamABCDE in complex with stalled folding intermediates. To do this we will use specific deuteration and neutron reflectometry in concert with previous PNR datasets of the BamABCDE complex bound to a gold surface to probe the structure of the Bam complex as it performs its function.

Identifier
DOI https://doi.org/10.5286/ISIS.E.83550836
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/83550836
Provenance
Creator Miss Nadisha Gamage; Miss Caitlin Hatton; Dr Luke Clifton; Mr Peter Wotherspoon; Dr Stephen Hall; Dr Tim Knowles; Dr Mark Jeeves
Publisher ISIS Neutron and Muon Source
Publication Year 2019
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2016-11-23T08:00:00Z
Temporal Coverage End 2016-11-28T08:00:00Z