Co-adsorption of monoclonal antibodies and non-ionic surfactants at the air/liquid interface

DOI

Nonionic surfactants usually do not show affinity to proteins and they work via competitive surface and interfacial processes in a binary mixture with protein. However, we show in this work that Tween 80, a much bigger nonionic molecule than C12E5, with an oleyl chain, could initiate interaction possibly via the hydrophobic effect, evident from the surface tension plots. We propose to examine their surface co-adsorption processes by neutron reflection to gain direct structural evidence of the interaction, as this is a rather new observation from such systems. We request 4 days of Inter beam time (weak signal and low Q range needed) to exploit the contrast variations by using deuterated Tween 80 in combination with 3 different water contrasts (weak signals under some contrast combinations). The proposed measurements will enable us to analyse the structures via the kinematic approach.

Identifier
DOI https://doi.org/10.5286/ISIS.E.81736412
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/81736412
Provenance
Creator Dr Chris van der Walle; Dr Mario Campana; Dr Peixun Li; Professor Jian Lu; Mr Robert Holman; Dr Lisa Pan; Dr John Webster; Dr Zongyi Li; Miss Daniela Ciumac; Mr Ruiheng Li
Publisher ISIS Neutron and Muon Source
Publication Year 2019
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Natural Sciences; Physics
Temporal Coverage Begin 2016-07-11T08:00:00Z
Temporal Coverage End 2016-07-13T08:00:00Z