Oxidative stress is a common harmful factor in broiler production, which can decreased immunity, feed intake, daily weight gain and quality of broilers . In addition, it also can damage intestinal barrier of poultry. It is particularly important to find ways to alleviate oxidative stress to birds. At present, many countries around the word have forbidden to add antibiotic growth promoters in poultry diets. Some studies have demonstrated great potential for natural plant-extracted as an alternative to antibiotic additives. L-theanine is a characteristic non-protein amino acid extracted from tea. It has many biological activities, including antioxidant, promoting animal growth and enhancing immunity etc. This study evaluated the effects of dietary L-theanine on growth performance, antioxidation, meatquality and intestinal micro flora in White Feather Broilers. The results showed that the effects of dietary 400 mg/kg L-theanine could improve the decline of growth performance, meat quality, enhance the intestinal mucosal barrier, antioxidant capacity and promote the composition of intestinal flora of broilers caused by acute oxidative stress. This experiment can provide experimental data reference for looking for feed additive products that can replace antibiotics. At the same time, it provides scientific theoretical reference for raising economic benefit of animal husbandry production.