Developing a general approach to resolution of accessory proteins bound to clathrin cages: auxilin as a model example.

DOI

Endocytosis plays a central role in allowing the selective transport of molecules, via membrane-derived vesicles, into cells. Vesicle formation is controlled by a complex network of proteins, some of which form a protein coat around the vesicle, giving these structures the name, ¿coated vesicles¿. We wish to understand how this network is formed in the case of clathrin-coated vesicle formation. To achieve this we need to visualise and quantify the nature of the interactions between clathrin and its accessory proteins. Since many accessory proteins adopt an extended conformation, visualising them bound to clathrin cages is problematic using other structural techniques. We aim to solve this problem using contrast variation SANS. Using auxilin binding to clathrin as a model system, we will develop a general approach to resolving accessory protein structures bound to clathrin cages.

Identifier
DOI https://doi.org/10.5286/ISIS.E.24088287
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24088287
Provenance
Creator Dr Cameron Neylon; Dr Corinne Smith; Mr Joe Jones
Publisher ISIS Neutron and Muon Source
Publication Year 2015
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2012-03-20T08:51:43Z
Temporal Coverage End 2012-10-20T08:13:26Z