Spotted sea bass is an important aquaculture species in China with high economic significance. The outbreaks of bacterial diseases, especially caused by N.seriolae, have occurred frequently due to intensive farming. With the high morbidity and mortality, fish nocardiosis is one of the most serious problems faced by the aquaculture industry of spotted sea bass. However, the regulatory mechanism of immune response in spotted sea bass after N.seriolae infection is still unclear. Our study could provide a new insight into the molecular regulatory mechanism of immune response after N. seriolae infection in spotted sea bass, and provides a basic guidance for the breeding of spotted sea bass with disease resistance in future.