Protein nanosheets assembled structures at the air-water interfaces for cell adhesion and culture

DOI

Cell adhesion and proliferation at the surface of liquids such as oil droplets is a surprising phenomenon as it is typically accepted in the field of bioengineering that cells require solid substrates to adhere and to exert mechanical forces required for their spreading and proliferation. Our laboratory recently reported such observations and uncovered that such phenomenon was mediated by a nanoscale (15-20 nm) mechanically strong protein film (nanosheet) assembled at the interface between the two liquids. However, little is known of the morphology and structure of such protein nanosheet and the impact of such structure on their mechanics, and in turn cell behaviour. This project aims to explore the structure of protein nanosheets assembled at analogous air-water interfaces, in situ.

Identifier
DOI https://doi.org/10.5286/ISIS.E.101134422
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/101134422
Provenance
Creator Dr Mario Campana; Mr Pengfei Liu; Miss Cardee Alexis; Dr Ali Zarbakhsh; Professor Julien Gautrot
Publisher ISIS Neutron and Muon Source
Publication Year 2022
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2019-02-06T09:00:00Z
Temporal Coverage End 2019-02-12T08:07:14Z