1- acylglycerol -3- phosphate -O- acyltransferase -2 (AGPAT2), an intermediate enzyme in triglyceride synthesis, is an important factor leading to lipodystrophy. In this study, antisense RNA was used to down-regulate agpat2 transcription and AGPAT2 protein expression. Studies have shown that antisense RNA technology can specifically knock down agpat2 in fish and cause abnormal lipid metabolism