Proteins of Different Size and Globular Stability Adsorbed at Oil/Water Interfaces Studied by Neutron Reflection

DOI

In this work we explore how to use neutron reflection to study protein adsorption at the oil/water interface. Inter is preferred because we have built technical capability on this instrument. Our specific aims are to (a) determine the actual thickness and volume fraction of globular lysozyme and (b) the effect of pH on the interfacial distribution. The results will enable us to compare the extent of proteins distributed into the oil region between lysozyme and large but less stable BSA at the interface. BSA has a distinct segment distribution in the oil region. The results from lysozyme will enable us to answer if this structural feature is unique to BSA as it contains regions for the delivery of fatty acids and lipids.

Identifier
DOI https://doi.org/10.5286/ISIS.E.61784198
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/61784198
Provenance
Creator Professor Jian Lu; Dr Mario Campana; Mr Zhiming Lu; Mr Ruiheng Li; Dr John Webster; Dr Cuixia Chen; Dr Zongyi Li
Publisher ISIS Neutron and Muon Source
Publication Year 2018
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Natural Sciences; Physics
Temporal Coverage Begin 2015-07-01T08:00:00Z
Temporal Coverage End 2015-07-03T08:00:00Z