Microbe abundance and biomass at station SEPT-1999-K6 in the Northeastern Aegean Sea

DOI

Borax-buffered formalin (final concentration 2% formaldehyde). Slides for nanoflagellates counting were stored at -20°C. Subsamples (10-30 ml) were concentrated on 25mm black polycarbonate filters of porosity 0.6µm for nanoflagellates, stained with DAPI for 10 min (Porter & Feig 1980) and filtered. Heterotrophic (HF) and photototrophic (PF) nanoflagellates were distinguished using UV and blue excitation and enumerated. Nanoflagellates were classified in size categories and the biovolume was calculated.Abundance data were converted into C biomass using 220fgC um-3 (Borsheim & Bratbak 1987) for nanoflagellates.Bacterial production was estimated by the 3H-leucine method (Kirchman et al. 1986, Kirchman 1993). At each depth, duplicate samples and a control were incubated with 1 nM L-[4,5 3H]-leucine (specific activity 128 Ci mmol-1) and 19 nM non radioactive leucine. Samples were incubated in the dark, at in situ temperature.

Identifier
DOI https://doi.org/10.1594/PANGAEA.688624
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.688624
Provenance
Creator Christaki, Urania ORCID logo
Publisher PANGAEA
Contributor Université du Littoral Côte d'Opale
Publication Year 2008
Funding Reference Fourth Framework Programme https://doi.org/10.13039/100011105 Crossref Funder ID MAS3970148 https://cordis.europa.eu/project/id/MAS3970148 Key coastal processes in the mesotropic Skagerrak and the oligotrophic Northern Aegean: a comparative study; Sixth Framework Programme https://doi.org/10.13039/100011103 Crossref Funder ID 36949 https://cordis.europa.eu/project/id/36949 Southern European Seas: Assessing and Modelling Ecosystem Changes
Rights Creative Commons Attribution 3.0 Unported; https://creativecommons.org/licenses/by/3.0/
OpenAccess true
Representation
Resource Type Dataset
Format text/tab-separated-values
Size 35 data points
Discipline Earth System Research
Spatial Coverage (25.750 LON, 39.977 LAT)