DNA sequencing of selected Globobulimina pseudospinescens organisms

We introduce FACIL (http://www.cmbi.ru.nl/FACIL), a fast, reliable tool to evaluate nucleic acid sequences for non-standard codes that detects alternative genetic codes even in species distantly related to known organisms. Results are visualized in a Genetic Code Logo. To illustrate the use of our method, we analysed several contigs derived from the mitochondrial genome of the foraminifer Globobulimina pseudospinescens. These are particularly challenging data, as the genome is highly fragmented and incomplete. Approximately 10,000 single-cell Globobulimina pseudospinescens organisms were isolated by hand from Gullmar Fjord Sweden sediment. After washing, total DNA was extracted and sequenced by Illumina sequencing. The reads were assembled using Edena. To illustrate the use of our method, we analysed several contigs derived from the mitochondrial genome of the foraminifer Globobulimina pseudospinescens, an organism without any sequenced relatives in the databases. These are particularly challenging data, as the genome is highly fragmented and incomplete. Overall design: DNA isolated from approximately 10,000 single-cell Globobulimina pseudospinescens organisms

Identifier
Source https://data.blue-cloud.org/search-details?step=~0121B48A7E47E202F189D6143F0B6EDC827E0C05F42
Metadata Access https://data.blue-cloud.org/api/collections/1B48A7E47E202F189D6143F0B6EDC827E0C05F42
Provenance
Instrument Illumina Genome Analyzer II; ILLUMINA
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Temporal Point 2011-06-21T00:00:00Z