The epiphytic microbiome assembly on the cosmopolitan marine diatom Thalassiosira rotula is niche-based, stable and reproducible.

With each cell division of phytoplankton, the newly established cell wall surface is immediately colonized by environmental bacteria, yet phytoplankton microbiomes are often highly specific. While phytoplankton-bacteria interactions play fundamental roles in marine ecosystems, namely for primary production, we have little understanding of the ecological paradigms behind epiphytic microbiome assembly. In a chance recolonization experiment, we exposed axenic diatoms of Thalassiosira rotula to several complex and compositionally different bacterial inocula obtained from conspecific, heterospecific and congeneric diatoms. The resulting microbiomes revealed a convergent assembly of highly diverse and compositionally different bacterial inocula containing up to 4406 operational taxonomic units (OTU) towards a consistent and reproducible T. rotula core microbiome consisting of 27 OTU, accounting for a total relative abundance of 94 +/- 4%. The microbiomes were dominated by Rhodobacteraceae (30.5%), Alteromonadaceae (27.7%), Oceanospirillales (18.5%) and were quantitatively most similar to its conspecific original. The high degree of overlap among the same OTU shared across the T. rotula acceptor microbiomes was indicative of a highly confined, stable and reproducible core microbiome. These observations reject bacterial colonization due to lottery theory and suggest a habitat filtering, niche-based microbiome assembly on the cosmopolitan marine bloom forming diatom T. rotula.

Identifier
Source https://data.blue-cloud.org/search-details?step=~01212E69A8269063C6829E7B508CCEA50093E47E3CB
Metadata Access https://data.blue-cloud.org/api/collections/12E69A8269063C6829E7B508CCEA50093E47E3CB
Provenance
Instrument Illumina MiSeq; ILLUMINA
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Spatial Coverage (7.900W, 54.184S, 7.900E, 54.184N)
Temporal Coverage Begin 2016-03-17T00:00:00Z
Temporal Coverage End 2019-06-07T00:00:00Z