High-throughput sequencing for life-history sorting and for bridging reference sequences in marine Gerromorpha (Insecta: Heteroptera)

Accurate information and association of larval specimens with adults is a major challenge in insect taxonomy. Fortunately, it is now possible for non-experts to sort specimen-rich bulk samples with DNA barcodes rapidly and cost-effectively. We demonstrate this process via nanopore barcoding of a collection of 757 marine insects (Insecta: Gerromorpha), of which 615 were nymphs and two of the six sampling localities did not have co-occurring male adults. We successfully associated 738 specimens to nine gerromorphan species, which would have been impossible with morphology alone. We also highlight two distinct, non-overlapping Gerromorpha COI sequence databases on GenBank, a consequence of using two different primer sets to amplify different regions of COI. This issue inevitably hinders species identifications with DNA-based methods, particularly for poorly represented groups such as marine insects. We bridged these databases by generating mitogenomes, where the full-length COI sequence is assembled as a by-product. We believe that this work will inspire future studies to incorporate DNA-based methods for more adult-larval association studies and for enhancing existing genetic resources, especially on understudied groups.

Identifier
Source https://data.blue-cloud.org/search-details?step=~012EEDCDE099209C8B1F563C913C2F83BA11CD6BD1E
Metadata Access https://data.blue-cloud.org/api/collections/EEDCDE099209C8B1F563C913C2F83BA11CD6BD1E
Provenance
Instrument Illumina HiSeq 4000; MinION; ILLUMINA; OXFORD_NANOPORE
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Temporal Coverage Begin 2018-09-05T00:00:00Z
Temporal Coverage End 2018-09-27T00:00:00Z