Fast scanning SAXS of living biological cells

DOI

We have recently employed hard X-ray scanning SAXS in a special fast scanning mode at ID13 to image hundreds of freeze-dried biological cells in a single scan, and have then transferred the method to fixed-hydrated cell, which was an important step towards more physiological conditions. In the proposed experiment, we suggest to now apply the method to living cells. We will fabricate special fluid chambers and investigate keratin-rich cells expressing particularly pronounced protein bundles, as a bench mark for studying such ordered structures.

Identifier
DOI https://doi.org/10.15151/ESRF-ES-940580063
Metadata Access https://icatplus.esrf.fr/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatplus.esrf.fr:inv/940580063
Provenance
Creator BORAM YU ORCID logo; Jan-Philipp BURCHERT ORCID logo; Jiliang LIU ORCID logo; Sophie AUGUST; Sarah KOESTER ORCID logo
Publisher ESRF (European Synchrotron Radiation Facility)
Publication Year 2025
Rights CC-BY-4.0; https://creativecommons.org/licenses/by/4.0
OpenAccess true
Representation
Resource Type Data from large facility measurement; Collection
Discipline Particles, Nuclei and Fields