Metagenomes of marine macroalgae, surrounding seawater and surrounding sediments

Here we analysed the microbial ecology of marine macroalgae, surrounding seawater, and surrounding sediment at Weihai, China (122.12 N, 37.56 E). Metagenomic sequencing of marine macroalgae, surrounding seawater, and surrounding sediment samples collected on 4 sampling dates in the year 2018-2019. For macroalgae, loosely attached microorganisms were removed by washing whole macroalgae thrice in flasks about 80% filled with sterile seawater on a shaker and collecting the washing suspensions in sterile bottles. Algae were then put in sterile seawater and remaining bacteria were removed by ultrasonication (2x 60s, 50-60 kHz). Suspensions with removed bacteria of each sample were subsequently pooled with the previously obtained washing suspensions. Then the washing suspension of each sample was filtered on 0.2 µm pore size polycarbonate membrane filters. Seawater samples were filtered on a 0.2 µm pore size polycarbonate membrane filters. For sediments, DNA was extracted from 300 mg sub-samples. Sequencing was done on the Illumina novaseq 6000 platform and 2x 150 base pair chemistry.

Identifier
Source https://data.blue-cloud.org/search-details?step=~012EE2FD165A98E262106CFDFD0048AE82C7BDFB8A2
Metadata Access https://data.blue-cloud.org/api/collections/EE2FD165A98E262106CFDFD0048AE82C7BDFB8A2
Provenance
Instrument Illumina NovaSeq 6000; ILLUMINA
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Spatial Coverage (122.121W, 37.562S, 122.121E, 37.562N)
Temporal Coverage Begin 2018-10-15T00:00:00Z
Temporal Coverage End 2019-08-01T00:00:00Z