This dataset contains the data used in the manuscript "Physiological adaptation of the diatom Pseudo-nitzschia delicatissima under copper starvation" accepted for publication in April 2023 in Marine Environmental Research. In the open ocean and particularly in iron (Fe)-limited environment, copper (Cu) deficiency might limit the growth of phytoplankton species. Cu is an essential trace metal used in electron-transfer reactions, such as respiration and photosynthesis, when bound to specific enzymes. Some phytoplankton species, such as the diatom Pseudo-nitzschia spp. can cope with Cu starvation through adaptative strategies. This dataset contains the data collected during the experimental starvation of a strain of the diatom P. delicatissima under laboratory controlled conditions.