This study reports the first chromosome-level genome assembly for Avicennia marina, the most widely distributed mangrove species of the world. This new assembly provides an excellent reference for phylogenomics and population genomics studies focused on A. marina and related species. Data here reported includes proximity ligation libraries Hi-C and Chicago, as well as resequencing data from 56 individuals. An annotation based on tissue-specific mRNA data of unprecedent quality is also provided, enabling functional identification of candidate genes. Overall, the data here reported enables a range of comparative genomics, molecular ecology, and functional divergence tools for the study of an ecological and socioeconomically relevant group of organisms.