Analysis of the genomic composition of phage YerA41

Genomic material isolated from purified phage YerA41 lysate was shown to contain RNA. YerA41 phage lysate was RNase treated to remove phage-external RNA and total RNA was then isolated from the phage preparate using Qiagen Rneasy mini kit. The isolated RNA was sequenced to elucidate its origin. The results suggested that the RNA originated from intact ribosomes of the host bacterium that contaminated the phage lysate. Overall design: RNA was isolated from two separate phage lysates in triplicate

Identifier
Source https://data.blue-cloud.org/search-details?step=~012C2420187341CD5D6334631CAED4A577B588AAEEF
Metadata Access https://data.blue-cloud.org/api/collections/C2420187341CD5D6334631CAED4A577B588AAEEF
Provenance
Instrument NextSeq 500; ILLUMINA
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Contributor Department of Bacteriology and Immunology, Medicum, Human Microbiome Research Program,, Faculty of Medicine, University of Helsinki
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Temporal Point 2022-04-09T00:00:00Z