Buffalo herd has its production concentrated in the Amazon region, specifically in the Marajo archipelago, state of Para. Buffalo milk stands out for its nutritional characteristics. In this context, studying this microbiome emerges as an interesting and underexplored topic in the literature, as well as whether certain biotic factors can influence milk colonization. The hypothesis that the buffalo milk microbiome could share taxa with samples from their feces, pasture soil, and even their calf's saliva was tested in this study. Additionally, a possible correlation between generations was also investigated. This study was conducted on a property in the Marajo archipelago, Amazon, Para Brazil. For this purpose, milk, feces, and saliva samples were collected in triplicate from first and third parity animals at their peak lactation. Pasture soil was also collected. Clinical examination of the mammary gland, macroscopic examination of milk secretion, and the California Mastitis. A Metabarcoding 16S DNA approach was used via the Illumina Miseq platform. The sequencing captured all species diversity present in the samples.