Structure of DNA nanostars gel as a function of ionic strength

DOI

DNA oligomers with properly designed sequences self-assemble into well defined constructs. Recently we exploited this methodology to produce bulk quantities of tetravalent DNA nanostars (each one composed of 196 nucleotides) and to explore the structural signatures of their aggregation process. We performed a SANS investigation focused on the evaluation of both the form factor and the temperature evolution of the scattered intensity at a nanostar concentration where the system forms a tetravalent equilibrium gel. The form factor was found to be in good agreement with molecular dynamics simulation and we could extract the effective structure factor and its evolution during the equilibrium gelation. On the basis of simulation results salt concentration have a strong impact on both the gel-transition and the DNA-NS gyration radius. Here we propose to investigate with SANS the effect of ionic strength.

Identifier
DOI https://doi.org/10.5291/ILL-DATA.9-13-695
Metadata Access https://data.ill.fr/openaire/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=10.5291/ILL-DATA.9-13-695
Provenance
Creator Paciaroni, Alessandro; Sciortino, Francesco; Castanon, Javier Fernandez; Porcar, Lionel; Comez, Lucia; Zanatta, Marco
Publisher Institut Laue-Langevin
Publication Year 2017
Rights OpenAccess; info:eu-repo/semantics/openAccess
OpenAccess true
Representation
Resource Type Dataset
Size 536 MB
Version 1
Discipline Particles, Nuclei and Fields