Single-cell RNA sequencing (scRNA-seq) are well suited for identifying the signature molecular markers or regulators in cells, this would benefit identifying or isolating various types of cells. Likewise, the signature transcriptome profile analysis at single cell level would well illustrate the key regulators or networks involved in gametogenesis and gonad development in animals. In this study, we analyzed the molecular signature of several distinct cell populations of Asian seabass adult ovaries through scRNA-seq.