This study examined the identity and potential ecology of the methane-related microbial community in an inactive SW Atlantic Carbonate-enriched Pockmark (SWACP), in the Brazilian SE Atlantic margin. Undisturbed sediment cores were enriched with CH4 with samples harvested at 16h, 120h, 240h, 720h, and 960h, followed by metataxonomics functional prediction, and the correlation of microbial groups with the incubation and enrichment types.