A SANS Study on the Influence of Shear on Peptide Fibrillisation

DOI

Time-resolved SANS will be performed to investigate the effect of shear on the fibrillisation of insulin following denaturation. The rheological response will be measured simultaneously. The measurements will be performed with the new Physica rheometer available on LOQ. The insulin will be thermally denatured at pD 2 in acid D2O to minimise background and to improve contrast. The kinetics of fibrillisation will be studied under shear at different rates and compared to the static fibrillisation kinetics. Shear at moderate shear rates is expected to enhance fibrillisation, although breakup of fibrils may be observed at higher shear rate. The project is related to the CCLRC Centre for Molecular-Structure and Design sponsored studentship in a collaboration between Ian Hamley (Reading), Steve King (ISIS) and Nick Terrill (Diamond)

Identifier
DOI https://doi.org/10.5286/ISIS.E.24003111
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24003111
Provenance
Creator Dr Adam Squires; Professor Ian Hamley; Miss Gemma Newby; Dr Valeria Castelletto
Publisher ISIS Neutron and Muon Source
Publication Year 2010
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Design; Fine Arts, Music, Theatre and Media Studies; Humanities
Temporal Coverage Begin 2007-12-04T10:24:00Z
Temporal Coverage End 2007-12-07T23:58:55Z