Climate change-induced lower and irregular rainfall, along with high evapotranspiration rates, contribute to soil salinization. This leads to reduced plant growth and yield losses, with rice (Oryza sativa), a staple crop, being particularly sensitive to salinization compared to other cereals. Developing resilient rice varieties is therefore imperative. Na+ transport and allocation are crucial in salt stress tolerance, with High-affinity K+ Transporter proteins (HKTs) playing a key role. Current methods to detect Na+ toxicity in plants, like inductively coupled plasma-mass-spectrometry, are destructive and lack a comprehensive Na+ profile at the cellular level, limiting our understanding of Na+ allocation across tissues. This project aims to profile Na+ at the cellular level in different rice varieties and also generate elemental profiles for Cl- and K+ to enhance our understanding of salinity responses.