This BAG application builds on the development of single particle cryo-EM in the Italian Structural Biology community, as in Italy there is no top-end instrument available. The groups in this application have acquired know-how in cryo.EM through international collaborations or by acquiring medium-level in-house equipment. Access to the CM01 beamline at the ESRF has been instrumental for the achievement of high-profile scientific results and publications in top journals. Presently three screening microscopes are available in Italy, these instruments will boost sample production and the number of applications to CM01. Given these premises a BAG from Italian institutions could ensure timely data collection, nurturing the expansion of the field in Italy and a high-level scientific production.