Bioturbators (such as bivalves, worms, polychaetes), living in a coastal area, experiences frequent changes in salinity. They are often exposed to mechanical disturbances (like wave, currents, storms) forcing them to bury deeper into the sediment to get a better foothold. In nature, these stressors often occurs simultaneously. Osmotic stress negatively affects the burial activity and the physiological performances of soft shell clam, Mya arenaria; however, the mechanism behind this is still unknown. In this dataset we present the combined effect of osmotic stress and repeated burrowing on the trypsin- and chymotrypsin-like proteasomal and lysosomal activity in gill tissue and digestive gland of Mya arenaria.