This dataset compiles the survival and sex-specific physiological responses of recovering F2 adult copepods following a five-day parental exposure (F1) to the isolated and combined effects of hypoxia and marine heatwave events in a laboratory set-up. In more detail, the data bundle includes: 1) Daily measurement of the physico-chemical parameters of the seawater for each aquarium holding the copepods in control conditions during the recovery period, 2) survival data corresponding to the percentage of F2 alive at the end of the recovery period, once they reached sexual maturity, 3) Sex-specific physiological data, in which routine metabolic rates (individual and specific), upper thermal limit, length, and dry weight are reported for F2 adult Acartia tonsa copepod following a 5-d parental exposure to the isolated and combined effects of hypoxia and marine heatwave events.

The experiment was conducted in the Marine Ecological and Evolutionary Physiology Laboratory (MEEP) at the University of Quebec in Rimouski (UQAR), Rimouski, QC, Canada. Copepods of the species Acartia tonsa were maintained following the stock culture culturing protocol described in Dam et al. 2021 (https://doi.org/10.1038/s41558-021-01131-5). Namely, copepods were kept in three 5 L aquaria filled with artificial seawater kept at a salinity of 28.4 ± 0.5. The physicochemical parameters of the seawater were the following: temperature of 18 ± 0.4 °C, oxygen saturation of 93.7 ± 6.0 %, and pH NBS of 8.20 ± 0.09 before the experiment. The photoperiod was kept at 13h light: 11 h dark. Copepods were fed ad libitum with a mixture of three phytoplankters: Tetraselmis sp., Thalassiosira weissflogii and Rhodomonas salina. Parents (F1) were transferred in 2 L aquaria placed in an experimental system during the 5-d exposure to the isolated and combined effects of hypoxia and MHW conditions. Once the parents (F1) were removed, their offspring (F2) were kept in the same 2 L aquaria and exposed to the control condition to simulate a recovery period until they reached sexual maturity. All traits were measured on F2 adult individuals. For all F1 traits measured during the exposure phase, please refer to the following database: https://doi.pangaea.de/10.1594/PANGAEA.967352.