Comparison of Nine Extraction Methods for Bacterial Identification Using the ONT MinION Sequencer

The purpose of this study was to identify a universal extraction method that could be coupled with ONT MinION sequencing for use in forensic situations for rapid identification of bacteria using the 16S FASTQ pipeline. It also evaluated the cloud-based data analysis software that is provided by ONT, EPI2ME. Eight test bacteria and nine extraction methods were tested.

Identifier
Source https://data.blue-cloud.org/search-details?step=~012577EA41DCE3B71BCF634400D53062E6F4B8AAED9
Metadata Access https://data.blue-cloud.org/api/collections/577EA41DCE3B71BCF634400D53062E6F4B8AAED9
Provenance
Instrument MinION; OXFORD_NANOPORE
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Contributor Sam Houston State University
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science