The PHYTOBS-MARCOBOLO dataset comprises long-term time series on marine microphytoplankton, from 2003 to 2021, along the entire French metropolitan coastline. Microphytoplankton data cover microscopic taxonomic identifications and counts. The PHYTOBS-MARCOBOLO dataset corresponds to a dataset extracted from the PHYTOBS network (DOI:10.17882/85178). The PHYTOBS network provides the scientific community and stakeholders with validated and qualified data on the biomass, abundance and composition of marine microphytoplankton in coastal and lagoon waters, with the aim of supporting scientific research. PHYTOBS-MARCOBOLO is a dataset used as part of the Horizon Europe MARCO-BOLO project (https://marcobolo-project.eu/), in which we are currently working to understand and analyze multi-decadal trends in coastal and marine biodiversity on a European scale. The PHYTOBS-MARCOBOLO dataset gathers data from 18 sampling sites, selected from the PHYTOBS-Network dataset according to requirements of time series quality and geographical location of sampling sites established as part of the MARCO-COLO project. This dataset was also formatted according to a template imposed for the European project.