Supplement table Ⅰ. Clinicopathological features of patients in our studySupplement table Ⅱ. Materials and methods of gene expression analysisSupplement table Ⅲ. Lists of differentially expressed genes in acral lentiginous melanoma compared to non-acral cutaneous melanomaSupplement table Ⅳ. Undirected and directed global significance scores of 18 pathways Supplement figure 1. Tumor immune-related gene expression profile of acral lentiginous melanoma (ALM) and non-acral cutaneous melanoma (NACM). Heatmap of unsupervised hierarchical clustering of total genes, MA plot, and principal component analysis plot showed that tumor immune-related genes were differently expressed between ALM and NACM, but ALM and NACM were not classified into separate clusters. Supplement figure 2. Volcano plots of each pathway that was significantly different in gene set enrichment analysis between acral lentiginous melanoma (ALM) and non-acral cutaneous melanoma (NACM). Whole genes are described as gray dots, while genes within the selected gene set are highlighted in yellow squares. Supplement figure 3. Immune cell profile analysis of acral lentiginous melanoma (ALM) and non-acral cutaneous melanoma (NACM). Pairwise box plots of immune-cell abundance scores obtained from the NanoString nSolver program and total score of tumor-infiltrating lymphocytes (TILs) according to melanoma subtype revealed that neutrophils were more abundant while total TILs were lowered in ALM.Supplement figure 4. Immune cell profile analysis using Cell-type Identification By Estimating Relative Subsets Of RNA Transcripts (CIBERSORT) of acral lentiginous melanoma (ALM) and non-acral cutaneous melanoma (NACM). Pairwise box plots of immune-cell scores revealed resting dendritic cells, neutrophils, and M2 macrophage subtypes were abundant in ALM than NACM.Supplement figure 5. Immunohistochemistry of acral lentiginous melanoma (ALM) and non-acral cutaneous melanoma (NACM). Representative histograms of ALM and positivity ratio of CXCL-5 /ENA-78, Cartilage oligometric matrix protein (COMP), LAG-3, and CD163 according to the melanoma subtype. (CXCL-5 /ENA-78, ×200; COMP, ×200; LAG3, ×100, CD163, ×200).ns: not significantSupplement figure 6. Gene expression profiles of normal human skin dataset (gene expression omnibus (GEO) accession number GSE5591, platform number GPL4213) according to anatomical site (acral versus non-acral). Volcano plot, adjusted p-value histogram, uniform manifold approximation and projection (UMAP), and MA plot revealed that there was no statistically significant difference (adjusted p-value <0.05) between acral and non-acral sites.

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