The dataset contains information obtained in a two-treatment (FWS vs. control) and two-period (1 vs. 2) crossover design study run in a commercial dairy herd from Argentina to test the hypothesis that follicular wave synchronization (FWS) before ovum pick-up (OPU) modifies follicle size and increases oocyte recovery and in vitro embryo development in beef cows and that short intersession interval OPU schemes, such as 14-day intersession interval, lead to potential carryover effects. The objectives were to evaluate: 1- the effect of FWS on follicle size, oocyte recovery, and in vitro embryo development in cows subjected to OPU sessions with an interval of 14 days, and 2- the probability of a carryover effect due to the short intersession interval used. The treatment had neither effect on follicle size nor the number of oocytes (P = 0.81 and 0.53, respectively). However, treatment tended to affect the total embryo number (P = 0.06) given that cows receiving FWS had twice the number of embryos as control cows. Finally, treatment did not affect the number of frozen embryos (P = 0.76). The period affected the number of good oocytes (P = 0.05). The period also tended to affect the number of viable oocytes given that it also increased in the second one (P = 0.07). Finally, the period tended to affect the total number of embryos given that it decreased in the second one (P = 0.10). Otherwise, treatment by period interaction (testing for carryover effect) affected the total number of follicles (P = 0.05) and, also, the number of viable oocytes (P = 0.02). It also tended to have effect on the number of < 6 mm follicles (P = 0.13), the total number of oocytes (P = 0.11), the number of good oocytes (P = 0.12), and the number of regular oocytes (P = 0.13).

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