The data include temperature proxy data (TEX86, MBT, brGMGT-I, NLR) and palynology (dinocyst assemblage data) for two coastal sections in the Australo-Antarctic Gulf. The stratigraphy of these sections is described in Frieling et al. (2018). The new data were generated to provide detailed multi-proxy temperature reconstructions for the region. A combination of organic geochemistry (lipid biomarker) analyses and palynology (mainly dinoflagellate cysts (dinocyst)) were conducted. A paper describing the results has been submitted (doi supplied when available). Sediment samples were acquired (by hand) during a field campaign to the coastal outcrop section at Point Margaret, Australia (-38.724667 N, 143.176389 E, 0m elevation) 14-28th of February 2016. Core samples from the Latrobe-1 borehole (-38.693056 N, 143.150000 E; drilled in 1963, ca. 20m elevation) were acquired from the geological survey core repository (Melbourne) during the same trip. From these sediment samples we quantified isoprenoid and branched glycerol dialkyl glycerol tetraethers (isoGDGTs) and branched glycerol monoalkyl glycerol tetraethers (brGMGTs) from organic total lipid extracts using ultra high performance liquid chromatography – mass spectrometry (UHPLC-MS) analyses. Furthermore, we used standard palynological techniques to obtain nearest living relative data from pollen and spore assemblages (methodology follows Huurdeman et al. 2021). Dinoflagellate cyst (dinocyst) assemblages were analysed from the same residues and here provided as relative abundances of ecogroups. All measurements and analyses were conducted in the organic geochemistry and palynology labs at Utrecht University, Netherlands between 2016 and 2018.