Microscopic images were captured with the Leica ICC50HD camera mounted on the Leica DM500 microscope, performed with fixed parameters in magnification 20x, and 56% horizontal and 40% vertical overlap. The rows and high-resolution images was generated using the captured images and an stitched algorithm using the homography matrices. The homography matrices were obtained from a small number of correspondences manually selected between each pair of images horizontal displacements and between pair of rows in vertical displacement. Microscope images PNG format format. High-resolution image PNG format , pixel size 0.3 µm, area 92.48 mm2. Homography matrices HDF5 files