In this study, bacterial communities derived from three different raw milks were serially propagated using two different culture systems, namely a monophasic culture system and an invert emulsion system. The resulting communities were characterized by enumeration and amplicon sequencing to assess the impact of the cultivation technique on their densities and structures in terms of alpha and beta diversity. Bacterial isolates were also identified using Sanger sequencing in order to evaluate the potential of the invert emulsion technique for bacterial enrichment and subsequent isolation.