Carapace properties of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification - files to run with R code

DOI

We examined the response of multiple structures used for predator defense in the California spiny lobster, Panulirus interruptus, to a series of ocean acidification-like conditions. Lobsters were collected by modified commercial traps offshore La Jolla, CA (in the area around 32.8534193, -117.2687516) in October 2016 and held at ambient conditions (pH 7.97, 16.5℃) before exposure to stable or diurnally fluctuating reduced pH conditions established by bubbling CO2 and as measured using best practices (ambient pH/stable, 7.97, 16.5℃; reduced pH/stable 7.67, 16.6℃; reduced pH with low fluctuations, 7.67 ± 0.05, 16.4℃; reduced pH with high fluctuations, 7.67 ± 0.10, 16.4℃). After three months, we examined the composition (wt. % and concentration of Ca and Mg), ultrastructure (cuticle and thickness of exocuticle ), and mechanical properties (hardness and stiffness). Layer thickness (µm) and wt. % mineralization were determined using a scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX). Concentration of elements (µmol/mg sample) was measured using inductively-coupled x-ray spectrometry (ICP-MS), while material properties (GPa) were measured using a nanoindenter with a Berkovitch diamond tip. The metadata file describes headers, units, methods, etc in each data file. CSV files are data files for separate measurements and will run with R code stored at GitHub to reproduce analyses and figures.

Identifier
DOI https://doi.org/10.1594/PANGAEA.945372
Related Identifier IsSupplementTo https://doi.org/10.3389/fmars.2022.909017
Related Identifier References https://doi.org/10.5281/zenodo.6596558
Related Identifier IsVariantFormOf https://doi.org/10.1594/PANGAEA.945362
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.945372
Provenance
Creator Lowder, Kaitlyn ORCID logo; deVries, Maya S ORCID logo; Hattingh, Ruan; Day, James M D; Andersson, Andreas J; Zerofski, Phillip; Taylor, Jennifer
Publisher PANGAEA
Publication Year 2022
Rights Creative Commons Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Representation
Resource Type Dataset
Format text/tab-separated-values
Size 5 data points
Discipline Earth System Research
Spatial Coverage (-117.269 LON, 32.853 LAT); off Southern California