This data are related with a study to describe the secretome of mesenchymal stromal cells derived from adipose tissue (AD-MSCs) of the cats and to evaluate the effects of culture conditions on conditioned medium proteomics by AD-MSCs. We used samples of adipose tissue collected from 5 queens. The samples were prepared to culture and assayed for osteogenic and adipogenic differentiation, and immunophenotypic analysis. Four culture conditions were tested, normoxia with fetal bovine serum (N + FBS - control), hypoxia with FBS (H + FBS), normoxia without FBS (N – FBS) and hypoxia without FBS (H – FBS). The secretome was collected and prepared to proteomics (ESI Q-TOF MS/MS).